Gain-of-function analysis of poplar CLE genes in Arabidopsis by exogenous application and over-expression assays.
Identifieur interne : 001870 ( Main/Exploration ); précédent : 001869; suivant : 001871Gain-of-function analysis of poplar CLE genes in Arabidopsis by exogenous application and over-expression assays.
Auteurs : Yisen Liu [République populaire de Chine] ; Shaohui Yang [République populaire de Chine] ; Yingjin Song [République populaire de Chine] ; Shuzhen Men [République populaire de Chine] ; Jiehua Wang [République populaire de Chine]Source :
- Journal of experimental botany [ 1460-2431 ] ; 2016.
Descripteurs français
- KwdFr :
- Alignement de séquences (MeSH), Analyse de profil d'expression de gènes (MeSH), Arabidopsis (croissance et développement), Arabidopsis (effets des médicaments et des substances chimiques), Arabidopsis (génétique), Faisceau vasculaire des plantes (effets des médicaments et des substances chimiques), Faisceau vasculaire des plantes (métabolisme), Famille multigénique (MeSH), Gènes de plante (MeSH), Motifs d'acides aminés (MeSH), Peptides (pharmacologie), Phylogenèse (MeSH), Phénotype (MeSH), Populus (génétique), Racines de plante (croissance et développement), Racines de plante (effets des médicaments et des substances chimiques), Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques), Spécificité d'organe (effets des médicaments et des substances chimiques), Spécificité d'organe (génétique), Végétaux génétiquement modifiés (MeSH).
- MESH :
- croissance et développement : Arabidopsis, Racines de plante.
- effets des médicaments et des substances chimiques : Arabidopsis, Faisceau vasculaire des plantes, Racines de plante, Régulation de l'expression des gènes végétaux, Spécificité d'organe.
- génétique : Arabidopsis, Populus, Spécificité d'organe.
- métabolisme : Faisceau vasculaire des plantes.
- pharmacologie : Peptides.
- Alignement de séquences, Analyse de profil d'expression de gènes, Famille multigénique, Gènes de plante, Motifs d'acides aminés, Phylogenèse, Phénotype, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Amino Acid Motifs (MeSH), Arabidopsis (drug effects), Arabidopsis (genetics), Arabidopsis (growth & development), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (drug effects), Genes, Plant (MeSH), Multigene Family (MeSH), Organ Specificity (drug effects), Organ Specificity (genetics), Peptides (pharmacology), Phenotype (MeSH), Phylogeny (MeSH), Plant Roots (drug effects), Plant Roots (growth & development), Plant Vascular Bundle (drug effects), Plant Vascular Bundle (metabolism), Plants, Genetically Modified (MeSH), Populus (genetics), Sequence Alignment (MeSH).
- MESH :
- chemical , pharmacology : Peptides.
- drug effects : Arabidopsis, Gene Expression Regulation, Plant, Organ Specificity, Plant Roots, Plant Vascular Bundle.
- genetics : Arabidopsis, Organ Specificity, Populus.
- growth & development : Arabidopsis, Plant Roots.
- metabolism : Plant Vascular Bundle.
- Amino Acid Motifs, Gene Expression Profiling, Genes, Plant, Multigene Family, Phenotype, Phylogeny, Plants, Genetically Modified, Sequence Alignment.
Abstract
Among 50 CLE gene family members in the Populus trichocarpa genome, three and six PtCLE genes encode a CLE motif sequence highly homologous to Arabidopsis CLV3 and TDIF peptides, respectively, which potentially make them functional equivalents. To test and compare their biological activity, we first chemically synthesized each dodecapeptide and analysed itsi n vitro bioactivity on Arabidopsis seedlings. Similarly, but to a different extent, three types of poplar CLV3-related peptides caused root meristem consumption, phyllotaxis disorder, anthocyanin accumulation and failure to enter the bolting stage. In comparison, application of two poplar TDIF-related peptides led to root length promotion in a dose-dependent manner with an even stronger effect observed for poplar TDIF-like peptide than TDIF. Next, we constructed CaMV35S:PtCLE transgenic plants for each of the nine PtCLE genes. Phenotypic abnormalities exemplified by arrested shoot apical meristem and abnormal flower structure were found to be more dominant and severe in 35S:PtCLV3 and 35S:PtCLV3-like2 lines than in the 35S:PtCLV3-like line. Disordered vasculature was detected in both stem and hypocotyl cross-sections in Arabidopsis plants over-expressing poplar TDIF-related genes with the most defective vascular patterning observed for TDIF2 and two TDIF-like genes. Phenotypic difference consistently observed in peptide application assay and transgenic analysis indicated the functional diversity of nine poplar PtCLE genes under investigation. This work represents the first report on the functional analysis of CLE genes in a tree species and constitutes a basis for further study of the CLE peptide signalling pathway in tree development.
DOI: 10.1093/jxb/erw045
PubMed: 26912800
Affiliations:
Links toward previous steps (curation, corpus...)
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Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Environmental Science and Engineering, Tianjin University, Tianjin 300072</wicri:regionArea><placeName><settlement type="city">Tianjin</settlement></placeName></affiliation></author><author><name sortKey="Men, Shuzhen" sort="Men, Shuzhen" uniqKey="Men S" first="Shuzhen" last="Men">Shuzhen Men</name><affiliation wicri:level="1"><nlm:affiliation>College of Life Sciences, Nankai University, Tianjin 300072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life Sciences, Nankai University, Tianjin 300072</wicri:regionArea><placeName><settlement type="city">Tianjin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Jiehua" sort="Wang, Jiehua" uniqKey="Wang J" first="Jiehua" last="Wang">Jiehua Wang</name><affiliation wicri:level="1"><nlm:affiliation>School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China jiehuawang@tju.edu.cn.</nlm:affiliation><country wicri:rule="url">République populaire de Chine</country><wicri:regionArea>School of Environmental Science and Engineering, Tianjin University, Tianjin 300072</wicri:regionArea><placeName><settlement type="city">Tianjin</settlement></placeName></affiliation></author></analytic><series><title level="j">Journal of experimental botany</title><idno type="eISSN">1460-2431</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Motifs (MeSH)</term><term>Arabidopsis (drug effects)</term><term>Arabidopsis (genetics)</term><term>Arabidopsis (growth & development)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Plant (drug effects)</term><term>Genes, Plant (MeSH)</term><term>Multigene Family (MeSH)</term><term>Organ Specificity (drug effects)</term><term>Organ Specificity (genetics)</term><term>Peptides (pharmacology)</term><term>Phenotype (MeSH)</term><term>Phylogeny (MeSH)</term><term>Plant Roots (drug effects)</term><term>Plant Roots (growth & development)</term><term>Plant Vascular Bundle (drug effects)</term><term>Plant Vascular Bundle (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (genetics)</term><term>Sequence Alignment (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences (MeSH)</term><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Arabidopsis (croissance et développement)</term><term>Arabidopsis (effets des médicaments et des substances chimiques)</term><term>Arabidopsis (génétique)</term><term>Faisceau vasculaire des plantes (effets des médicaments et des substances chimiques)</term><term>Faisceau vasculaire des plantes (métabolisme)</term><term>Famille multigénique (MeSH)</term><term>Gènes de plante (MeSH)</term><term>Motifs d'acides aminés (MeSH)</term><term>Peptides (pharmacologie)</term><term>Phylogenèse (MeSH)</term><term>Phénotype (MeSH)</term><term>Populus (génétique)</term><term>Racines de plante (croissance et développement)</term><term>Racines de plante (effets des médicaments et des substances chimiques)</term><term>Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques)</term><term>Spécificité d'organe (effets des médicaments et des substances chimiques)</term><term>Spécificité d'organe (génétique)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Peptides</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Arabidopsis</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Arabidopsis</term><term>Gene Expression Regulation, Plant</term><term>Organ Specificity</term><term>Plant Roots</term><term>Plant Vascular Bundle</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Arabidopsis</term><term>Faisceau vasculaire des plantes</term><term>Racines de plante</term><term>Régulation de l'expression des gènes végétaux</term><term>Spécificité d'organe</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term><term>Organ Specificity</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Arabidopsis</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arabidopsis</term><term>Populus</term><term>Spécificité d'organe</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Vascular Bundle</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Faisceau vasculaire des plantes</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Peptides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Motifs</term><term>Gene Expression Profiling</term><term>Genes, Plant</term><term>Multigene Family</term><term>Phenotype</term><term>Phylogeny</term><term>Plants, Genetically Modified</term><term>Sequence Alignment</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Analyse de profil d'expression de gènes</term><term>Famille multigénique</term><term>Gènes de plante</term><term>Motifs d'acides aminés</term><term>Phylogenèse</term><term>Phénotype</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Among 50 CLE gene family members in the Populus trichocarpa genome, three and six PtCLE genes encode a CLE motif sequence highly homologous to Arabidopsis CLV3 and TDIF peptides, respectively, which potentially make them functional equivalents. To test and compare their biological activity, we first chemically synthesized each dodecapeptide and analysed itsi n vitro bioactivity on Arabidopsis seedlings. Similarly, but to a different extent, three types of poplar CLV3-related peptides caused root meristem consumption, phyllotaxis disorder, anthocyanin accumulation and failure to enter the bolting stage. In comparison, application of two poplar TDIF-related peptides led to root length promotion in a dose-dependent manner with an even stronger effect observed for poplar TDIF-like peptide than TDIF. Next, we constructed CaMV35S:PtCLE transgenic plants for each of the nine PtCLE genes. Phenotypic abnormalities exemplified by arrested shoot apical meristem and abnormal flower structure were found to be more dominant and severe in 35S:PtCLV3 and 35S:PtCLV3-like2 lines than in the 35S:PtCLV3-like line. Disordered vasculature was detected in both stem and hypocotyl cross-sections in Arabidopsis plants over-expressing poplar TDIF-related genes with the most defective vascular patterning observed for TDIF2 and two TDIF-like genes. Phenotypic difference consistently observed in peptide application assay and transgenic analysis indicated the functional diversity of nine poplar PtCLE genes under investigation. This work represents the first report on the functional analysis of CLE genes in a tree species and constitutes a basis for further study of the CLE peptide signalling pathway in tree development.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26912800</PMID><DateCompleted><Year>2016</Year><Month>12</Month><Day>13</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1460-2431</ISSN><JournalIssue CitedMedium="Internet"><Volume>67</Volume><Issue>8</Issue><PubDate><Year>2016</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J Exp Bot</ISOAbbreviation></Journal><ArticleTitle>Gain-of-function analysis of poplar CLE genes in Arabidopsis by exogenous application and over-expression assays.</ArticleTitle><Pagination><MedlinePgn>2309-24</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/jxb/erw045</ELocationID><Abstract><AbstractText>Among 50 CLE gene family members in the Populus trichocarpa genome, three and six PtCLE genes encode a CLE motif sequence highly homologous to Arabidopsis CLV3 and TDIF peptides, respectively, which potentially make them functional equivalents. To test and compare their biological activity, we first chemically synthesized each dodecapeptide and analysed itsi n vitro bioactivity on Arabidopsis seedlings. Similarly, but to a different extent, three types of poplar CLV3-related peptides caused root meristem consumption, phyllotaxis disorder, anthocyanin accumulation and failure to enter the bolting stage. In comparison, application of two poplar TDIF-related peptides led to root length promotion in a dose-dependent manner with an even stronger effect observed for poplar TDIF-like peptide than TDIF. Next, we constructed CaMV35S:PtCLE transgenic plants for each of the nine PtCLE genes. Phenotypic abnormalities exemplified by arrested shoot apical meristem and abnormal flower structure were found to be more dominant and severe in 35S:PtCLV3 and 35S:PtCLV3-like2 lines than in the 35S:PtCLV3-like line. Disordered vasculature was detected in both stem and hypocotyl cross-sections in Arabidopsis plants over-expressing poplar TDIF-related genes with the most defective vascular patterning observed for TDIF2 and two TDIF-like genes. Phenotypic difference consistently observed in peptide application assay and transgenic analysis indicated the functional diversity of nine poplar PtCLE genes under investigation. This work represents the first report on the functional analysis of CLE genes in a tree species and constitutes a basis for further study of the CLE peptide signalling pathway in tree development.</AbstractText><CopyrightInformation>© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Yisen</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Shaohui</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Yingjin</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Men</LastName><ForeName>Shuzhen</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>College of Life Sciences, Nankai University, Tianjin 300072, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Jiehua</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China jiehuawang@tju.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>02</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010455">Peptides</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020816" MajorTopicYN="N">Amino Acid Motifs</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005810" MajorTopicYN="N">Multigene Family</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009928" MajorTopicYN="N">Organ Specificity</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010455" MajorTopicYN="N">Peptides</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058526" MajorTopicYN="N">Plant Vascular Bundle</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">CLE gene family</Keyword><Keyword MajorTopicYN="N">meristem</Keyword><Keyword MajorTopicYN="N">peptide application</Keyword><Keyword MajorTopicYN="N">poplar</Keyword><Keyword MajorTopicYN="N">transgenic Arabidopsis</Keyword><Keyword MajorTopicYN="N">vasculature.</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>2</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>2</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26912800</ArticleId><ArticleId IdType="pii">erw045</ArticleId><ArticleId IdType="doi">10.1093/jxb/erw045</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Tianjin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Liu, Yisen" sort="Liu, Yisen" uniqKey="Liu Y" first="Yisen" last="Liu">Yisen Liu</name></noRegion><name sortKey="Men, Shuzhen" sort="Men, Shuzhen" uniqKey="Men S" first="Shuzhen" last="Men">Shuzhen Men</name><name sortKey="Song, Yingjin" sort="Song, Yingjin" uniqKey="Song Y" first="Yingjin" last="Song">Yingjin Song</name><name sortKey="Wang, Jiehua" sort="Wang, Jiehua" uniqKey="Wang J" first="Jiehua" last="Wang">Jiehua Wang</name><name sortKey="Yang, Shaohui" sort="Yang, Shaohui" uniqKey="Yang S" first="Shaohui" last="Yang">Shaohui Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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